An engineered human albumin enhances half-life and transmucosal delivery when fused to protein-based biologics

Malin Bern; Jeannette Nilsen; Mattia Ferrarese; Kine M. K. Sand; Torleif T. Gjølberg; Heidrun E. Lode; Robert J. Davidson; Rodney M. Camire; Espen S. Bækkevold; Stian Foss; Algirdas Grevys; Bjørn Dalhus; John Wilson; Lene S. Høydahl; Gregory J. Christianson; Derry C. Roopenian; Tilman Schlothauer; Terje E. Michaelsen; Morten C. Moe; Silvia Lombardi; Mirko Pinotti; Inger Sandlie; Alessio Branchini; Jan Terje Andersen

doi:10.1126/scitranslmed.abb0580

Research ArticleDrug Delivery

An engineered human albumin enhances half-life and transmucosal delivery when fused to protein-based biologics

View ORCID ProfileMalin Bern ¹,²,
View ORCID ProfileJeannette Nilsen ¹,²,*,
View ORCID ProfileMattia Ferrarese³,*,
View ORCID ProfileKine M. K. Sand¹,²,⁴,*,
Torleif T. Gjølberg¹,²,⁵,†,
View ORCID ProfileHeidrun E. Lode ¹,²,⁵,†,
Robert J. Davidson⁶,
Rodney M. Camire⁶,⁷,
Espen S. Bækkevold⁸,
View ORCID ProfileStian Foss ¹,²,
View ORCID ProfileAlgirdas Grevys¹,²,
View ORCID ProfileBjørn Dalhus⁹,
John Wilson¹⁰,
View ORCID ProfileLene S. Høydahl¹,¹¹,
View ORCID ProfileGregory J. Christianson¹⁰,
View ORCID ProfileDerry C. Roopenian¹⁰,
Tilman Schlothauer¹²,
View ORCID ProfileTerje E. Michaelsen¹³,¹⁴,
View ORCID ProfileMorten C. Moe⁵,
View ORCID ProfileSilvia Lombardi³,
View ORCID ProfileMirko Pinotti ³,
Inger Sandlie¹,⁴,
View ORCID ProfileAlessio Branchini ³,‡ and
View ORCID ProfileJan Terje Andersen ¹,²,‡

¹Centre for Immune Regulation (CIR) and Department of Immunology, University of Oslo and Oslo University Hospital, Rikshospitalet, 0372 Oslo, Norway.
²Institute of Clinical Medicine and Department of Pharmacology, University of Oslo and Oslo University Hospital, 0372 Oslo, Norway.
³Department of Life Sciences and Biotechnology and LTTA, University of Ferrara, 44121 Ferrara, Italy.
⁴CIR and Department of Biosciences, University of Oslo, 0371 Oslo, Norway.
⁵Department of Ophthalmology, University of Oslo and Oslo University Hospital, Rikshospitalet, 0450 Oslo, Norway.
⁶The Children’s Hospital of Philadelphia, The Raymond G. Perelman Center for Cellular and Molecular Therapeutics, Philadelphia, PA 19104, USA.
⁷Department of Pediatrics, Division of Hematology, University of Pennsylvania, Philadelphia, PA 19104, USA.
⁸CIR and Department of Pathology, University of Oslo and Oslo University Hospital, Rikshospitalet, 0372 Oslo, Norway.
⁹Department for Medical Biochemistry, Institute for Clinical Medicine and Department for Microbiology, Clinic for Laboratory Medicine, University of Oslo, 0372 Oslo, Norway.
¹⁰The Jackson Laboratory, Bar Harbor, ME 04609, USA.
¹¹KG Jebsen Coeliac Disease Research Centre, University of Oslo, 0372 Oslo, Norway.
¹²Biochemical and Analytical Research, Large Molecule Research, Roche Pharma Research and Early Development (pRED), Roche Innovation Center Munich, 82377 Penzberg, Germany.
¹³Department of Infectious Disease Immunology, Norwegian Institute of Public Health, 0456 Oslo, Norway.
¹⁴Department of Chemical Pharmacy, School of Pharmacy, University of Oslo, 0371 Oslo, Norway.

↵‡Corresponding author. Email: j.t.andersen{at}medisin.uio.no (J.T.A.); brnlss{at}unife.it (A.B.).

↵* These authors contributed equally to this work.
↵† These authors contributed equally to this work.

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Science Translational Medicine 14 Oct 2020:
Vol. 12, Issue 565, eabb0580
DOI: 10.1126/scitranslmed.abb0580

Malin Bern

1Centre for Immune Regulation (CIR) and Department of Immunology, University of Oslo and Oslo University Hospital, Rikshospitalet, 0372 Oslo, Norway.

2Institute of Clinical Medicine and Department of Pharmacology, University of Oslo and Oslo University Hospital, 0372 Oslo, Norway.

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ORCID record for Malin Bern

Jeannette Nilsen

1Centre for Immune Regulation (CIR) and Department of Immunology, University of Oslo and Oslo University Hospital, Rikshospitalet, 0372 Oslo, Norway.

2Institute of Clinical Medicine and Department of Pharmacology, University of Oslo and Oslo University Hospital, 0372 Oslo, Norway.

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Mattia Ferrarese

3Department of Life Sciences and Biotechnology and LTTA, University of Ferrara, 44121 Ferrara, Italy.

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Kine M. K. Sand

1Centre for Immune Regulation (CIR) and Department of Immunology, University of Oslo and Oslo University Hospital, Rikshospitalet, 0372 Oslo, Norway.

2Institute of Clinical Medicine and Department of Pharmacology, University of Oslo and Oslo University Hospital, 0372 Oslo, Norway.

4CIR and Department of Biosciences, University of Oslo, 0371 Oslo, Norway.

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Torleif T. Gjølberg

1Centre for Immune Regulation (CIR) and Department of Immunology, University of Oslo and Oslo University Hospital, Rikshospitalet, 0372 Oslo, Norway.

2Institute of Clinical Medicine and Department of Pharmacology, University of Oslo and Oslo University Hospital, 0372 Oslo, Norway.

5Department of Ophthalmology, University of Oslo and Oslo University Hospital, Rikshospitalet, 0450 Oslo, Norway.

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Heidrun E. Lode

1Centre for Immune Regulation (CIR) and Department of Immunology, University of Oslo and Oslo University Hospital, Rikshospitalet, 0372 Oslo, Norway.

2Institute of Clinical Medicine and Department of Pharmacology, University of Oslo and Oslo University Hospital, 0372 Oslo, Norway.

5Department of Ophthalmology, University of Oslo and Oslo University Hospital, Rikshospitalet, 0450 Oslo, Norway.

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Robert J. Davidson

6The Children’s Hospital of Philadelphia, The Raymond G. Perelman Center for Cellular and Molecular Therapeutics, Philadelphia, PA 19104, USA.

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Rodney M. Camire

6The Children’s Hospital of Philadelphia, The Raymond G. Perelman Center for Cellular and Molecular Therapeutics, Philadelphia, PA 19104, USA.

7Department of Pediatrics, Division of Hematology, University of Pennsylvania, Philadelphia, PA 19104, USA.

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Espen S. Bækkevold

8CIR and Department of Pathology, University of Oslo and Oslo University Hospital, Rikshospitalet, 0372 Oslo, Norway.

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Stian Foss

1Centre for Immune Regulation (CIR) and Department of Immunology, University of Oslo and Oslo University Hospital, Rikshospitalet, 0372 Oslo, Norway.

2Institute of Clinical Medicine and Department of Pharmacology, University of Oslo and Oslo University Hospital, 0372 Oslo, Norway.

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Algirdas Grevys

1Centre for Immune Regulation (CIR) and Department of Immunology, University of Oslo and Oslo University Hospital, Rikshospitalet, 0372 Oslo, Norway.

2Institute of Clinical Medicine and Department of Pharmacology, University of Oslo and Oslo University Hospital, 0372 Oslo, Norway.

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Bjørn Dalhus

9Department for Medical Biochemistry, Institute for Clinical Medicine and Department for Microbiology, Clinic for Laboratory Medicine, University of Oslo, 0372 Oslo, Norway.

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John Wilson

10The Jackson Laboratory, Bar Harbor, ME 04609, USA.

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Lene S. Høydahl

1Centre for Immune Regulation (CIR) and Department of Immunology, University of Oslo and Oslo University Hospital, Rikshospitalet, 0372 Oslo, Norway.

11KG Jebsen Coeliac Disease Research Centre, University of Oslo, 0372 Oslo, Norway.

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Gregory J. Christianson

10The Jackson Laboratory, Bar Harbor, ME 04609, USA.

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Derry C. Roopenian

10The Jackson Laboratory, Bar Harbor, ME 04609, USA.

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Tilman Schlothauer

12Biochemical and Analytical Research, Large Molecule Research, Roche Pharma Research and Early Development (pRED), Roche Innovation Center Munich, 82377 Penzberg, Germany.

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Terje E. Michaelsen

13Department of Infectious Disease Immunology, Norwegian Institute of Public Health, 0456 Oslo, Norway.

14Department of Chemical Pharmacy, School of Pharmacy, University of Oslo, 0371 Oslo, Norway.

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Morten C. Moe

5Department of Ophthalmology, University of Oslo and Oslo University Hospital, Rikshospitalet, 0450 Oslo, Norway.

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Silvia Lombardi

3Department of Life Sciences and Biotechnology and LTTA, University of Ferrara, 44121 Ferrara, Italy.

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Mirko Pinotti

3Department of Life Sciences and Biotechnology and LTTA, University of Ferrara, 44121 Ferrara, Italy.

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Inger Sandlie

1Centre for Immune Regulation (CIR) and Department of Immunology, University of Oslo and Oslo University Hospital, Rikshospitalet, 0372 Oslo, Norway.

4CIR and Department of Biosciences, University of Oslo, 0371 Oslo, Norway.

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Alessio Branchini

3Department of Life Sciences and Biotechnology and LTTA, University of Ferrara, 44121 Ferrara, Italy.

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For correspondence: j.t.andersen@medisin.uio.no brnlss@unife.it

Jan Terje Andersen

1Centre for Immune Regulation (CIR) and Department of Immunology, University of Oslo and Oslo University Hospital, Rikshospitalet, 0372 Oslo, Norway.

2Institute of Clinical Medicine and Department of Pharmacology, University of Oslo and Oslo University Hospital, 0372 Oslo, Norway.

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For correspondence: j.t.andersen@medisin.uio.no brnlss@unife.it

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An engineered human albumin enhances half-life and transmucosal delivery when fused to protein-based biologics

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