Antibody signature induced by SARS-CoV-2 spike protein immunogens in rabbits

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Science Translational Medicine  01 Jul 2020:
Vol. 12, Issue 550, eabc3539
DOI: 10.1126/scitranslmed.abc3539

Fig. S1. Purified SARS-CoV-2 proteins analyzed by SDS–polyacrylamide gel electrophoresis under reducing and nonreducing conditions.

Fig. S2. Anti-spike reactivity of post-vaccination rabbit sera measured by ELISA.

Fig. S3. Isotyping of rabbit serum binding to the SARS-CoV-2 spike protein after immunization.

Fig. S4. Steady-state equilibrium analysis of serum antibody binding by SPR.

Fig. S5. Neutralizing activity of post-vaccination rabbit serum antibodies in a pseudovirion neutralization assay.

Fig. S6. Sequence alignment of spike protein from diverse CoV strains.

Fig. S7. Structural representation of antigenic sites identified in SARS-CoV-2 using GFPDL.

Table S1. Sequence conservation of antigenic sites among different CoV strains.

Data file S1. Primary data.