Research ArticlePulmonary Hypertension

ACTRIIA-Fc rebalances activin/GDF versus BMP signaling in pulmonary hypertension

See allHide authors and affiliations

Science Translational Medicine  13 May 2020:
Vol. 12, Issue 543, eaaz5660
DOI: 10.1126/scitranslmed.aaz5660

You are currently viewing the abstract.

View Full Text

Log in to view the full text

Log in through your institution

Log in through your institution

Remedying pulmonary vascular remodeling

Alterations in bone morphogenetic protein, growth and differentiation factor (GDF), and transforming growth factor–β signaling contribute to vascular remodeling and elevated pulmonary blood pressure in pulmonary arterial hypertension (PAH) and experimental pulmonary hypertension (PH). Yung et al. used a fusion protein to inhibit activin A/B and GDF8/11, ligands increased in PAH and PH tissues that signal through ACTRIIA to promote maladaptive vascular cell phenotypes. Treating rodent models of PH with the fusion protein reduced pulmonary artery pressure, pulmonary arteriolar muscularization and occlusion, right ventricular hypertrophy, and cell proliferation in the lungs. Results suggest that the fusion protein may help treat PH by reversing pathologic vascular remodeling.

Abstract

Human genetics, biomarker, and animal studies implicate loss of function in bone morphogenetic protein (BMP) signaling and maladaptive transforming growth factor–β (TGFβ) signaling as drivers of pulmonary arterial hypertension (PAH). Although sharing common receptors and effectors with BMP/TGFβ, the function of activin and growth and differentiation factor (GDF) ligands in PAH are less well defined. Increased expression of GDF8, GDF11, and activin A was detected in lung lesions from humans with PAH and experimental rodent models of pulmonary hypertension (PH). ACTRIIA-Fc, a potent GDF8/11 and activin ligand trap, was used to test the roles of these ligands in animal and cellular models of PH. By blocking GDF8/11- and activin-mediated SMAD2/3 activation in vascular cells, ACTRIIA-Fc attenuated proliferation of pulmonary arterial smooth muscle cells and pulmonary microvascular endothelial cells. In several experimental models of PH, prophylactic administration of ACTRIIA-Fc markedly improved hemodynamics, right ventricular (RV) hypertrophy, RV function, and arteriolar remodeling. When administered after the establishment of hemodynamically severe PH in a vasculoproliferative model, ACTRIIA-Fc was more effective than vasodilator in attenuating PH and arteriolar remodeling. Potent antiremodeling effects of ACTRIIA-Fc were associated with inhibition of SMAD2/3 activation and downstream transcriptional activity, inhibition of proliferation, and enhancement of apoptosis in the vascular wall. ACTRIIA-Fc reveals an unexpectedly prominent role of GDF8, GDF11, and activin as drivers of pulmonary vascular disease and represents a therapeutic strategy for restoring the balance between SMAD1/5/9 and SMAD2/3 signaling in PAH.

View Full Text

Stay Connected to Science Translational Medicine