Research ArticleCancer
Enhanced detection of circulating tumor DNA by fragment size analysis
- View ORCID ProfileFlorent Mouliere1,2,*,†,
- View ORCID ProfileDineika Chandrananda1,2,*,
- View ORCID ProfileAnna M. Piskorz1,2,*,
- View ORCID ProfileElizabeth K. Moore1,2,3,*,
- James Morris1,2,
- Lise Barlebo Ahlborn4,5,
- Richard Mair1,2,6,
- View ORCID ProfileTeodora Goranova1,2,
- View ORCID ProfileFrancesco Marass1,2,7,8,
- View ORCID ProfileKatrin Heider1,2,
- View ORCID ProfileJonathan C. M. Wan1,2,
- Anna Supernat1,2,9,
- View ORCID ProfileIrena Hudecova1,2,
- Ioannis Gounaris1,2,3,
- Susana Ros1,2,
- Mercedes Jimenez-Linan2,3,
- Javier Garcia-Corbacho10,
- Keval Patel1,2,
- Olga Østrup5,
- Suzanne Murphy1,2,
- View ORCID ProfileMatthew D. Eldridge1,2,
- View ORCID ProfileDavina Gale1,2,
- View ORCID ProfileGrant D. Stewart2,3,11,
- Johanna Burge2,11,
- View ORCID ProfileWendy N. Cooper1,2,
- Michiel S. van der Heijden12,13,
- View ORCID ProfileCharles E. Massie1,2,14,
- View ORCID ProfileColin Watts15,
- View ORCID ProfilePippa Corrie3,
- Simon Pacey3,14,
- View ORCID ProfileKevin M. Brindle1,2,16,
- View ORCID ProfileRichard D. Baird17,
- View ORCID ProfileMorten Mau-Sørensen4,
- Christine A. Parkinson1,2,3,18,19,
- View ORCID ProfileChristopher G. Smith1,2,
- View ORCID ProfileJames D. Brenton1,2,3,18,19,‡,§ and
- View ORCID ProfileNitzan Rosenfeld1,2,‡,§
- 1Cancer Research UK Cambridge Institute, University of Cambridge, CB2 0RE Cambridge, UK.
- 2Cancer Research UK Major Centre–Cambridge, Cancer Research UK Cambridge Institute, CB2 0RE Cambridge, UK.
- 3Cambridge University Hospitals NHS Foundation Trust, CB2 0QQ Cambridge, UK.
- 4Department of Oncology, Rigshospitalet, Copenhagen University Hospital, DK-2100 Copenhagen, Denmark.
- 5Centre for Genomic Medicine, Rigshospitalet, Copenhagen University Hospital, DK-2100 Copenhagen, Denmark.
- 6Division of Neurosurgery, Department of Clinical Neurosciences, University of Cambridge, CB2 0QQ Cambridge, UK.
- 7Department of Biosystems Science and Engineering, ETH Zurich, 4058 Basel, Switzerland.
- 8Swiss Institute of Bioinformatics, 4058 Basel, Switzerland.
- 9Department of Medical Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of Gdańsk, 80-211 Gdańsk, Poland.
- 10Clinical Trials Unit, Clinic Institute of Haematological and Oncological Diseases, Hospital Clinic de Barcelona, 170 08036 Barcelona, Spain.
- 11Academic Urology Group, Department of Surgery, University of Cambridge, CB2 0QQ Cambridge, UK.
- 12Division of Molecular Carcinogenesis, Netherlands Cancer Institute, 1066 CX Amsterdam, Netherlands.
- 13Department of Medical Oncology, Netherlands Cancer Institute, 1066 CX Amsterdam, Netherlands.
- 14Department of Oncology, University of Cambridge, CB2 0XZ Cambridge, UK.
- 15Institute of Cancer Genomics Science, University of Birmingham, B15 2TT Birmingham, UK.
- 16Department of Biochemistry, University of Cambridge, CB2 1QW Cambridge, UK.
- 17Early Phase Clinical Trials and Breast Cancer Research Teams, Cancer Research UK Cambridge Centre, CB2 0QQ Cambridge, UK.
- 18Department of Oncology, Hutchison/MRC Research Centre, University of Cambridge, CB2 0XZ Cambridge, UK.
- 19NIHR Cambridge Biomedical Research Centre, CB2 0QQ Cambridge, UK.
- ↵§Corresponding author. Email: james.brenton{at}cruk.cam.ac.uk (J.D.B.); nitzan.rosenfeld{at}cruk.cam.ac.uk (N.R.)
↵* These authors contributed equally to this work as co-first authors.
↵† Present address: Amsterdam UMC, Vrije Universiteit Amsterdam, Department of Pathology, Cancer Center Amsterdam, de Boelelaan 1117, 1081 HV Amsterdam, Netherlands.
↵‡ These authors contributed equally to this work as co-senior authors.
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Science Translational Medicine 07 Nov 2018:
Vol. 10, Issue 466, eaat4921
DOI: 10.1126/scitranslmed.aat4921
Vol. 10, Issue 466, eaat4921
DOI: 10.1126/scitranslmed.aat4921
Florent Mouliere
1Cancer Research UK Cambridge Institute, University of Cambridge, CB2 0RE Cambridge, UK.
2Cancer Research UK Major Centre–Cambridge, Cancer Research UK Cambridge Institute, CB2 0RE Cambridge, UK.
Dineika Chandrananda
1Cancer Research UK Cambridge Institute, University of Cambridge, CB2 0RE Cambridge, UK.
2Cancer Research UK Major Centre–Cambridge, Cancer Research UK Cambridge Institute, CB2 0RE Cambridge, UK.
Anna M. Piskorz
1Cancer Research UK Cambridge Institute, University of Cambridge, CB2 0RE Cambridge, UK.
2Cancer Research UK Major Centre–Cambridge, Cancer Research UK Cambridge Institute, CB2 0RE Cambridge, UK.
Elizabeth K. Moore
1Cancer Research UK Cambridge Institute, University of Cambridge, CB2 0RE Cambridge, UK.
2Cancer Research UK Major Centre–Cambridge, Cancer Research UK Cambridge Institute, CB2 0RE Cambridge, UK.
3Cambridge University Hospitals NHS Foundation Trust, CB2 0QQ Cambridge, UK.
James Morris
1Cancer Research UK Cambridge Institute, University of Cambridge, CB2 0RE Cambridge, UK.
2Cancer Research UK Major Centre–Cambridge, Cancer Research UK Cambridge Institute, CB2 0RE Cambridge, UK.
Lise Barlebo Ahlborn
4Department of Oncology, Rigshospitalet, Copenhagen University Hospital, DK-2100 Copenhagen, Denmark.
5Centre for Genomic Medicine, Rigshospitalet, Copenhagen University Hospital, DK-2100 Copenhagen, Denmark.
Richard Mair
1Cancer Research UK Cambridge Institute, University of Cambridge, CB2 0RE Cambridge, UK.
2Cancer Research UK Major Centre–Cambridge, Cancer Research UK Cambridge Institute, CB2 0RE Cambridge, UK.
6Division of Neurosurgery, Department of Clinical Neurosciences, University of Cambridge, CB2 0QQ Cambridge, UK.
Teodora Goranova
1Cancer Research UK Cambridge Institute, University of Cambridge, CB2 0RE Cambridge, UK.
2Cancer Research UK Major Centre–Cambridge, Cancer Research UK Cambridge Institute, CB2 0RE Cambridge, UK.
Francesco Marass
1Cancer Research UK Cambridge Institute, University of Cambridge, CB2 0RE Cambridge, UK.
2Cancer Research UK Major Centre–Cambridge, Cancer Research UK Cambridge Institute, CB2 0RE Cambridge, UK.
7Department of Biosystems Science and Engineering, ETH Zurich, 4058 Basel, Switzerland.
8Swiss Institute of Bioinformatics, 4058 Basel, Switzerland.
Katrin Heider
1Cancer Research UK Cambridge Institute, University of Cambridge, CB2 0RE Cambridge, UK.
2Cancer Research UK Major Centre–Cambridge, Cancer Research UK Cambridge Institute, CB2 0RE Cambridge, UK.
Jonathan C. M. Wan
1Cancer Research UK Cambridge Institute, University of Cambridge, CB2 0RE Cambridge, UK.
2Cancer Research UK Major Centre–Cambridge, Cancer Research UK Cambridge Institute, CB2 0RE Cambridge, UK.
Anna Supernat
1Cancer Research UK Cambridge Institute, University of Cambridge, CB2 0RE Cambridge, UK.
2Cancer Research UK Major Centre–Cambridge, Cancer Research UK Cambridge Institute, CB2 0RE Cambridge, UK.
9Department of Medical Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of Gdańsk, 80-211 Gdańsk, Poland.
Irena Hudecova
1Cancer Research UK Cambridge Institute, University of Cambridge, CB2 0RE Cambridge, UK.
2Cancer Research UK Major Centre–Cambridge, Cancer Research UK Cambridge Institute, CB2 0RE Cambridge, UK.
Ioannis Gounaris
1Cancer Research UK Cambridge Institute, University of Cambridge, CB2 0RE Cambridge, UK.
2Cancer Research UK Major Centre–Cambridge, Cancer Research UK Cambridge Institute, CB2 0RE Cambridge, UK.
3Cambridge University Hospitals NHS Foundation Trust, CB2 0QQ Cambridge, UK.
Susana Ros
1Cancer Research UK Cambridge Institute, University of Cambridge, CB2 0RE Cambridge, UK.
2Cancer Research UK Major Centre–Cambridge, Cancer Research UK Cambridge Institute, CB2 0RE Cambridge, UK.
Mercedes Jimenez-Linan
2Cancer Research UK Major Centre–Cambridge, Cancer Research UK Cambridge Institute, CB2 0RE Cambridge, UK.
3Cambridge University Hospitals NHS Foundation Trust, CB2 0QQ Cambridge, UK.
Javier Garcia-Corbacho
10Clinical Trials Unit, Clinic Institute of Haematological and Oncological Diseases, Hospital Clinic de Barcelona, 170 08036 Barcelona, Spain.
Keval Patel
1Cancer Research UK Cambridge Institute, University of Cambridge, CB2 0RE Cambridge, UK.
2Cancer Research UK Major Centre–Cambridge, Cancer Research UK Cambridge Institute, CB2 0RE Cambridge, UK.
Olga Østrup
5Centre for Genomic Medicine, Rigshospitalet, Copenhagen University Hospital, DK-2100 Copenhagen, Denmark.
Suzanne Murphy
1Cancer Research UK Cambridge Institute, University of Cambridge, CB2 0RE Cambridge, UK.
2Cancer Research UK Major Centre–Cambridge, Cancer Research UK Cambridge Institute, CB2 0RE Cambridge, UK.
Matthew D. Eldridge
1Cancer Research UK Cambridge Institute, University of Cambridge, CB2 0RE Cambridge, UK.
2Cancer Research UK Major Centre–Cambridge, Cancer Research UK Cambridge Institute, CB2 0RE Cambridge, UK.
Davina Gale
1Cancer Research UK Cambridge Institute, University of Cambridge, CB2 0RE Cambridge, UK.
2Cancer Research UK Major Centre–Cambridge, Cancer Research UK Cambridge Institute, CB2 0RE Cambridge, UK.
Grant D. Stewart
2Cancer Research UK Major Centre–Cambridge, Cancer Research UK Cambridge Institute, CB2 0RE Cambridge, UK.
3Cambridge University Hospitals NHS Foundation Trust, CB2 0QQ Cambridge, UK.
11Academic Urology Group, Department of Surgery, University of Cambridge, CB2 0QQ Cambridge, UK.
Johanna Burge
2Cancer Research UK Major Centre–Cambridge, Cancer Research UK Cambridge Institute, CB2 0RE Cambridge, UK.
11Academic Urology Group, Department of Surgery, University of Cambridge, CB2 0QQ Cambridge, UK.
Wendy N. Cooper
1Cancer Research UK Cambridge Institute, University of Cambridge, CB2 0RE Cambridge, UK.
2Cancer Research UK Major Centre–Cambridge, Cancer Research UK Cambridge Institute, CB2 0RE Cambridge, UK.
Michiel S. van der Heijden
12Division of Molecular Carcinogenesis, Netherlands Cancer Institute, 1066 CX Amsterdam, Netherlands.
13Department of Medical Oncology, Netherlands Cancer Institute, 1066 CX Amsterdam, Netherlands.
Charles E. Massie
1Cancer Research UK Cambridge Institute, University of Cambridge, CB2 0RE Cambridge, UK.
2Cancer Research UK Major Centre–Cambridge, Cancer Research UK Cambridge Institute, CB2 0RE Cambridge, UK.
14Department of Oncology, University of Cambridge, CB2 0XZ Cambridge, UK.
Colin Watts
15Institute of Cancer Genomics Science, University of Birmingham, B15 2TT Birmingham, UK.
Pippa Corrie
3Cambridge University Hospitals NHS Foundation Trust, CB2 0QQ Cambridge, UK.
Simon Pacey
3Cambridge University Hospitals NHS Foundation Trust, CB2 0QQ Cambridge, UK.
14Department of Oncology, University of Cambridge, CB2 0XZ Cambridge, UK.
Kevin M. Brindle
1Cancer Research UK Cambridge Institute, University of Cambridge, CB2 0RE Cambridge, UK.
2Cancer Research UK Major Centre–Cambridge, Cancer Research UK Cambridge Institute, CB2 0RE Cambridge, UK.
16Department of Biochemistry, University of Cambridge, CB2 1QW Cambridge, UK.
Richard D. Baird
17Early Phase Clinical Trials and Breast Cancer Research Teams, Cancer Research UK Cambridge Centre, CB2 0QQ Cambridge, UK.
Morten Mau-Sørensen
4Department of Oncology, Rigshospitalet, Copenhagen University Hospital, DK-2100 Copenhagen, Denmark.
Christine A. Parkinson
1Cancer Research UK Cambridge Institute, University of Cambridge, CB2 0RE Cambridge, UK.
2Cancer Research UK Major Centre–Cambridge, Cancer Research UK Cambridge Institute, CB2 0RE Cambridge, UK.
3Cambridge University Hospitals NHS Foundation Trust, CB2 0QQ Cambridge, UK.
18Department of Oncology, Hutchison/MRC Research Centre, University of Cambridge, CB2 0XZ Cambridge, UK.
19NIHR Cambridge Biomedical Research Centre, CB2 0QQ Cambridge, UK.
Christopher G. Smith
1Cancer Research UK Cambridge Institute, University of Cambridge, CB2 0RE Cambridge, UK.
2Cancer Research UK Major Centre–Cambridge, Cancer Research UK Cambridge Institute, CB2 0RE Cambridge, UK.
James D. Brenton
1Cancer Research UK Cambridge Institute, University of Cambridge, CB2 0RE Cambridge, UK.
2Cancer Research UK Major Centre–Cambridge, Cancer Research UK Cambridge Institute, CB2 0RE Cambridge, UK.
3Cambridge University Hospitals NHS Foundation Trust, CB2 0QQ Cambridge, UK.
18Department of Oncology, Hutchison/MRC Research Centre, University of Cambridge, CB2 0XZ Cambridge, UK.
19NIHR Cambridge Biomedical Research Centre, CB2 0QQ Cambridge, UK.
Nitzan Rosenfeld
1Cancer Research UK Cambridge Institute, University of Cambridge, CB2 0RE Cambridge, UK.
2Cancer Research UK Major Centre–Cambridge, Cancer Research UK Cambridge Institute, CB2 0RE Cambridge, UK.
