In situ identification of Gram-negative bacteria in human lungs using a topical fluorescent peptide targeting lipid A

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Science Translational Medicine  24 Oct 2018:
Vol. 10, Issue 464, eaal0033
DOI: 10.1126/scitranslmed.aal0033

Lung infection in real time

Lung infections are frequent causes of complications in mechanically ventilated and immunosuppressed patients. However, the diagnosis is challenging, requires risky procedures, and is time consuming. Now, Akram et al. have developed an imaging method that is able to detect Gram-negative bacteria in real time in the distal part of the human lung. Using a fluorescent probe binding to lipid A, a molecule expressed on Gram-negative bacterial membranes, in combination with an optical endomicroscope, the researchers rapidly detected Gram-negative infections in distal airways in hospitalized individuals. The results suggest that the approach could accelerate the diagnosis of bacterial lung infection and facilitate the evaluation of antibiotic treatment efficacy.

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