Research ArticleImaging

An optical nanoreporter of endolysosomal lipid accumulation reveals enduring effects of diet on hepatic macrophages in vivo

See allHide authors and affiliations

Science Translational Medicine  03 Oct 2018:
Vol. 10, Issue 461, eaar2680
DOI: 10.1126/scitranslmed.aar2680

Enlightening endolysosomal lipids

Lipid accumulation contributes to multiple diseases including atherosclerosis, nonalcoholic fatty liver disease (NAFLD), and lysosomal storage diseases. To noninvasively measure lipids in vivo, Galassi et al. engineered a carbon nanotube optical reporter for endolysosomal organelle uptake. In mouse models of lysosomal storage disease and NAFLD, the nanoreporter’s near-infrared fluorescence tracked lipid accumulation within Kupffer cells (liver macrophages) and revealed that switching mice from a high-fat, high-fructose diet to standard chow only partially reversed liver lipid accumulation. This optical imaging agent could help to elucidate mechanisms of lipid accumulation in disease or be useful for drug development testing.

View Full Text

Stay Connected to Science Translational Medicine