PerspectiveInfectious Disease

Toward a Systems-Level Analysis of Infection Biology: A New Method for Conducting Genetic Screens in Human Cells

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Science Translational Medicine  16 Dec 2009:
Vol. 1, Issue 11, pp. 11ps13
DOI: 10.1126/scitranslmed.3000550

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Loss-of-function genetic screens have facilitated great strides in our understanding of the biology of model organisms but have not been possible in diploid human cells. A recent report by Brummelkamp’s group in Science describes the use of insertional mutagenesis to generate loss-of-function alleles in a largely haploid human cell line and demonstrates the versatility of this method in screens designed to investigate the host/pathogen interaction. This approach has strengths that are complementary to existing strategies and will facilitate progress toward a systems-level understanding of infectious disease and ultimately the development of new therapeutics.


  • Citation: S. A. Stanley and D. T. Hung, Toward a systems-level analysis of infection biology: A new method for conducting genetic screens in human cells. Sci. Transl. Med. 1, 11ps13 (2009).

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