Supplementary Materials

This PDF file includes:

  • Materials and Methods
  • Fig. S1. In vitro responses of cultured neurons to touch demonstrate the efficiency of the mosaic knockout strategy.
  • Fig. S2. Mechanosensory phenotypes in adult PiezocKO mice.
  • Fig. S3. Custom-built devices for mechanical stimulation of the mouse cheek.
  • Fig. S4. Piezo2 is required for trigeminal responses to vibration but not heat.
  • Fig. S5. CFA induces chronic inflammation and sensitization to heat but not pinch.
  • Fig. S6. Responses to gentle mechanical stimuli require Piezo2 in the CFA inflammatory pain model.
  • Fig. S7. Controls for brush responses after capsaicin-induced inflammation.
  • Fig. S8. Responses to gentle mechanical stimuli require Piezo2 in the spared nerve injury neuropathic pain model.
  • Fig. S9. Four individuals with loss-of-function mutations in PIEZO2.
  • Fig. S10. Psychophysical evaluation of PIEZO2LOF individuals reveals diagnostic mechanosensory deficits.

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