Supplementary Materials

The PDF file includes:

  • Fig. S1. Effects of EP on human MPP cells.
  • Fig. S2. Effects of EP on cell cycling of HSCs.
  • Fig. S3. Alterations of iron-associated gene expression in human hematopoietic cells after EP treatment.
  • Fig. S4. TPO-R–independent stimulation of HSC self-renewal by EP.
  • Fig. S5. Reduction of HSCs in c-Mpl−/− mice.
  • Fig. S6. Complete blood counts of c-Mpl−/− mice after in vivo treatment with EP.
  • Fig. S7. Alterations of glycolysis-associated genes in mouse HSCs after EP treatment.
  • Fig. S8. FAC dose finding for ex vivo assays.
  • Fig. S9. Intracellular ROS concentrations in HSCs after EP treatment.
  • Fig. S10. Iron-dependent HSC self-renewal upon chelator treatment.
  • Fig. S11. Iron-associated gene expression changes in ITP patients treated with EP.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). DEGs in TPO-treated human HSCs.
  • Table S2 (Microsoft Excel format). DEGs in EP-treated human HSCs.
  • Table S3 (Microsoft Excel format). Commonly altered DEGs between EP- and TPO-treated human HSCs.
  • Table S4 (Microsoft Excel format). IPA analysis with DEGs specifically detected in EP-treated human HSCs.
  • Table S5 (Microsoft Excel format). Plasma concentrations of EP in mice.
  • Table S6 (Microsoft Excel format). DEGs in EP-treated mouse HSCs.
  • Table S7 (Microsoft Excel format). IPA analysis with DEGs in EP-treated mouse HSCs.
  • Table S8 (Microsoft Excel format). Altered metabolites detected in EP-treated HPC7 cells.
  • Table S9 (Microsoft Excel format). Pathway analysis with altered metabolites detected in EP-treated HPC7 cells.
  • Table S10 (Microsoft Excel format). IPA analysis with altered metabolites detected in EP-treated HPC7 cells.
  • Table S11 (Microsoft Excel format). Information on primary human-derived specimens.
  • Table S12 (Microsoft Excel format). List of antibodies used in FACS experiments.
  • Table S13 (Microsoft Excel format). List of primers for qPCR.