Supplementary Materials

Supplementary Material for:

Staphylococcus aureus α toxin potentiates opportunistic bacterial lung infections

Taylor S. Cohen, Jamese J. Hilliard, Omari Jones-Nelson, Ashley E. Keller, Terrence O'Day, Christine Tkaczyk, Antonio DiGiandomenico, Melissa Hamilton, Mark Pelletier, Qun Wang, Binh An Diep, Vien T. M. Le, Lily Cheng, JoAnn Suzich, C. Kendall Stover, Bret R. Sellman*

*Corresponding author. E-mail: sellmanb{at}medimmune.com

Published 9 March 2016, Sci. Transl. Med. 8, 329ra31 (2016)
DOI: 10.1126/scitranslmed.aad9922

This PDF file includes:

  • Materials and Methods
  • Fig. S1. MEDI4893* protects against mixed infection with diverse S. aureus strains.
  • Fig. S2. MEDI4893* prevents mortality associated with mixed infection of S. aureus and either K. pneumoniae or A. baumannii.
  • Fig. S3. AT potentiates infection with either K. pneumoniae or A. baumannii.
  • Fig. S4. Mixed infection does not result in excessive tissue damage or inflammation.
  • Fig. S5. Immune cell populations in the lung.
  • Fig. S6. AT neutralization increases phagocytosis of S. aureus by AMs.
  • Fig. S7. AT reduces phagocytosis of S. aureus by AMs.
  • Fig. S8. Anti-NK1.1 depletion of NK cells.
  • Fig. S9. AT prevents colocalization of S. aureus with acidic lysosomes.
  • Fig. S10. AT reduces lysosomal acidification in human cells.
  • Fig. S11. Contribution of immune cells to the clearance of P. aeruginosa or K. pneumoniae from the lung.
  • Fig. S12. Confocal imaging analysis of P. aeruginosa internalization by hPBMCs.
  • Fig. S13. Flow cytometry gating strategy and isotype controls.
  • Table S1. Exact P values for CFU data in Fig. 1.
  • Table S2. Bacterial strains.

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Other Supplementary Material for this manuscript includes the following:

  • Table S3 Source data for all figures (Excel).