Supplementary Materials

Supplementary Material for:

A sand fly salivary protein vaccine shows efficacy against vectortransmitted cutaneous leishmaniasis in nonhuman primates

Fabiano Oliveira, Edgar Rowton, Hamide Aslan, Regis Gomes, Philip A. Castrovinci, Patricia H. Alvarenga, Maha Abdeladhim, Clarissa Teixeira, Claudio Meneses, Lindsey T. Kleeman, Anderson B. Guimarães-Costa, Tobin E. Rowland, Dana Gilmore, Seydou Doumbia, Steven G. Reed, Phillip G. Lawyer, John F. Andersen, Shaden Kamhawi,* Jesus G. Valenzuela*

*Corresponding author. E-mail: jvalenzuela{at}niaid.nih.gov (J.G.V.); skamhawi{at}niaid.nih.gov (S.K.)

Published 3 June 2015, Sci. Transl. Med. 7, 290ra90 (2015)
DOI: 10.1126/scitranslmed.aaa3043

This PDF file includes:

  • Fig. S1. Disease progression of CL in NHP after transmission of L. major by bites of 50 infected P. duboscqi sand flies.
  • Fig. S2. CD3+ lymphocytes are the main source of Leishmania-specific IFN-γ in NHP exposed to uninfected P. duboscqi sand flies.
  • Fig. S3. CD8+ lymphocytes are not critical for protection from CL in NHP exposed to uninfected sand fly bites.
  • Fig. S4. Anti-PdSP15 antibodies and CD8+ lymphocytes are not critical for protection from CL in PdSP15-immunized NHP.
  • Fig. S5. The crystal structure of PdSP15.
  • Table S1. Measurements (mm) of skin indurations 48 hours after inoculation with plasmids coding for selected sand fly salivary proteins.
  • Table S2. IFN-γ and IL-4 mRNA expression by RT-qPCR.
  • Table S3. Data collection and refinement statistics for PdSP15.

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Other Supplementary Material for this manuscript includes the following:

  • Source data. Excel file

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