Rapid pathogen-specific phenotypic antibiotic susceptibility testing using digital LAMP quantification in clinical samples

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Science Translational Medicine  04 Oct 2017:
Vol. 9, Issue 410, eaal3693
DOI: 10.1126/scitranslmed.aal3693

Rapidly recognizing resistance

Reducing the time required to determine whether a bacterial sample is resistant to an antibiotic could hasten proper treatment of infections. Toward this goal, Schoepp et al. developed an antibiotic susceptibility test that could be performed within 30 min using clinical urine samples. The test uses digital loop-mediated isothermal amplification to measure the amount of nucleic acid markers of antibiotic susceptibility produced by bacteria present within a clinical sample after a brief incubation with an antibiotic. Performing the test on a microfluidic platform enabled single-molecule amplification and quantification in real time, determining Escherichia coli susceptibility comparably to gold standard methods, but in less time.