Responsive monitoring of mitochondrial redox states in heart muscle predicts impending cardiac arrest

See allHide authors and affiliations

Science Translational Medicine  20 Sep 2017:
Vol. 9, Issue 408, eaan0117
DOI: 10.1126/scitranslmed.aan0117

Monitoring mitochondria to predict cardiac dysfunction

Mitochondria adapt to changing environmental conditions to try to meet the energy needs of a cell. Perry et al. used resonance Raman spectroscopy to monitor the mitochondrial redox state in the heart. Under low oxygen conditions, electron transport chain cytochromes within the mitochondria become progressively more reduced, which is detected on the basis of the spectral signal. The authors identified a threshold value that predicted the onset of cardiac arrest in rats under hypoxemic conditions and showed that they could monitor mitochondrial redox state during myocardial ischemia-reperfusion in pigs. Although the analysis was restricted to the heart, this approach could be useful for monitoring perfusion and viability of other tissues.