Research ArticleParkinson’s Disease

Lentiviral Overexpression of GRK6 Alleviates l-Dopa–Induced Dyskinesia in Experimental Parkinson’s Disease

Science Translational Medicine  21 Apr 2010:
Vol. 2, Issue 28, pp. 28ra28
DOI: 10.1126/scitranslmed.3000664

You are currently viewing the abstract.

View Full Text

Via your Institution

Log in through your institution

Log in through your institution


Abstract

Parkinson’s disease is caused primarily by degeneration of brain dopaminergic neurons in the substantia nigra and the consequent deficit of dopamine in the striatum. Dopamine replacement therapy with the dopamine precursor l-dopa is the mainstay of current treatment. After several years, however, the patients develop l-dopa–induced dyskinesia, or abnormal involuntary movements, thought to be due to excessive signaling via dopamine receptors. G protein–coupled receptor kinases (GRKs) control desensitization of dopamine receptors. We found that dyskinesia is attenuated by lentivirus-mediated overexpression of GRK6 in the striatum in rodent and primate models of Parkinson’s disease. Conversely, reduction of GRK6 concentration by microRNA delivered with lentiviral vector exacerbated dyskinesia in parkinsonian rats. GRK6 suppressed dyskinesia in monkeys without compromising the antiparkinsonian effects of l-dopa and even prolonged the antiparkinsonian effect of a lower dose of l-dopa. Our finding that increased availability of GRK6 ameliorates dyskinesia and increases duration of the antiparkinsonian action of l-dopa suggests a promising approach for controlling both dyskinesia and motor fluctuations in Parkinson’s disease.

Footnotes

  • * These authors contributed equally to this work.

  • Citation: M. R. Ahmed, A. Berthet, E. Bychkov, G. Porras, Q. Li, B. H. Bioulac, Y. T. Carl, B. Bloch, S. Kook, I. Aubert, S. Dovero, E. Doudnikoff, V. V. Gurevich, E. V. Gurevich, E. Bezard, Lentiviral Overexpression of GRK6 Alleviates l-Dopa–Induced Dyskinesia in Experimental Parkinson's Disease Sci. Transl. Med. 2, 28ra28 (2010).

View Full Text