Research ArticleEMERGING INFECTIONS

A conserved transcriptional response to intranasal Ebola virus exposure in nonhuman primates prior to onset of fever

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Science Translational Medicine  28 Mar 2018:
Vol. 10, Issue 434, eaaq1016
DOI: 10.1126/scitranslmed.aaq1016

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An enlightening Ebola model

Using large doses of highly pathogenic agents in animal models can ensure consistent and fully penetrant infection but does not recapitulate human exposure and possibly immune responses. Speranza and colleagues intranasally infected cynomolgus macaques with a small dose of Ebola virus, which led to various disease presentation and allowed for detection of early immunity. Regardless of the timing of symptoms, analysis of the host response revealed a conserved interferon signature that preceded fever by several days. These results shed light on the host response to Ebola virus and established a model that could be used for future studies of pathogenesis, treatment, or prevention.

Abstract

Ebola virus disease (EVD), caused by Ebola virus (EBOV), is a severe illness characterized by case fatality rates of up to 90%. The sporadic nature of outbreaks in resource-limited areas has hindered the ability to characterize the pathogenesis of EVD at all stages of infection but particularly early host responses. Pathogenesis is often studied in nonhuman primate (NHP) models of disease that replicate major aspects of human EVD. Typically, NHP models use a large infectious dose, are carried out through intramuscular or aerosol exposure, and have a fairly uniform disease course. By contrast, we report our analysis of the host response to EBOV after intranasal exposure. Twelve cynomolgus macaques were infected with 100 plaque-forming units of EBOV/Makona through intranasal exposure and presented with varying times to onset of EVD. We used RNA sequencing and a newly developed NanoString CodeSet to monitor the host response via changes in RNA transcripts over time. When individual animal gene expression data were phased based on the onset of sustained fever, the first clinical sign of severe disease, mathematical models indicated that interferon-stimulated genes appeared as early as 4 days before fever onset. This demonstrates that lethal EVD has a uniform and predictable response to infection regardless of time to onset. Furthermore, expression of a subset of genes could predict disease development before other host-based indications of infection such as fever.

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